Figure 2 | Scientific Reports

Figure 2

From: Identification of CD38, CD97, and CD278 on the HIV surface using a novel flow virometry screening assay

Figure 2

Screening viruses from primary cells using a novel flow virometry assay. (A) Schematic depicting the experimental workflow used for staining PBMC viruses. Blood samples from four uninfected donors were used to isolate PBMC. Four different HIV isolates (BaL, IIIB, SF162, BG505) were propagated in PBMC. Each viral stock was then stained individually with the full panel of LEGENDScreen antibodies before being diluted with PBS, and then acquired on the cytometer. Data are displayed in a heatmap with darker shades representing higher levels of staining. (B) Heat map displaying the mean fluorescence (PE MESF) generated from each of the stained PBMC virus isolates that were tested (columns 2–5). Each row represents a different antibody stain from the LEGENDScreen panel, and each column represents a different viral isolate. Staining intensities for proteins of interest were subtracted from the mean staining values generated from staining with matched isotype controls. Samples with staining below the isotype control are shown in white. *Staining intensities from HIVIIIB produced in the H9 T cell line are shown in column one, whereas columns 2–5 show viral isolates propagated in PBMC. (C) A subset of antigens from (B) that were previously established to be present on HIV particles are labelled in (C), shown here as positive controls for assay specificity.

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