Figure 12

Co-treatment of TNF-α and IFN-γ Induces Cell Death. (A) Representative images of cell death in L02 after 72 h of the indicated treatments, measured by PI stain. Red indicates dead cells. Scale bar, 50 μm. (B) Quantification of the cell death in (A). (C–D). L02 cells were stimulated with the following concentrations of cytokines, TNF-α (12.5, 25, and 50 ng/ml)/IFN-γ (25, 50, and 100 ng/ml) for 12, 24, 36, 48, 60, and 72 h, measured by CCK8.