Figure 2

The effects of boron on cell cycle and proliferation rate before and after PI3K/Akt inhibition. Flow cytometry was used to analyze the effects of boron on the phases of G0/G1, S, G2/M and proliferation rate of IEC-6 cells before and after PI3K and Akt were inhibited by LY294002 and MK-2206 2HCL, respectively. (A) (Flow chart) (a–c) IEC-6 cultured without LY294002 or MK-2206 2HCL; (d–f) IEC-6 cultured with 10 μmol/L LY294002; (g–i) IEC-6 cultured with 10 μmol/L MK-2206 2HCL; 0 mmol/L boron (a, d, g), 0.8 mmol/ L boron (b, e, h) and 40 mmol/L boron (c, f, i); (B)The changes in the ratio of G0 phase cell number to G1 phase cell number before and after the addition of LY249002; (C)The changes of cell number in S phase before and after the addition of LY249002; (D)The changes in the ratio of G2 phase cell number to M phase cell number before and after the addition of LY249002; (E) The IEC-6 proliferation rate before and after LY294002 addition; (F)The changes in the ratio of G0 phase cell number to G1 phase cell number before and after the addition of MK-2206 2HCL; (G)The changes of cell number in S phase before and after the addition of MK-2206 2HCL; (H)The changes in the ratio of G2 phase cell number to M phase cell number before and after the addition of MK-2206 2HCL; (I) The IEC-6 proliferation rate before and after MK2206 2HCL addition.Values are presented as the mean ± SD; Mean values with asterisk (*) differed significantly (p < 0.05), mean values with two asterisks (**) indicate extremely significant differences (p < 0.01).