Figure 3 | Scientific Reports

Figure 3

From: PI3K/Akt signaling pathway mediates the effect of low-dose boron on barrier function, proliferation and apoptosis in rat intestinal epithelial cells

Figure 3

The effects of boron on apoptosis rate before and after PI3K/Akt inhibition. Flow cytometry and CCK-8 were used to analyze the effect of boron on the apoptosis rate of IEC-6 cells before and after PI3K and Akt were inhibited by LY294002 and MK-2206 2HCL, respectively. (A) Flow chart for IEC-6 apoptosis detection before and after MK-2206 2HCL addition. (a–c) IEC-6 cultured without LY294002 or MK-2206 2HCL; (d–f) IEC-6 cultured with 10 μmol/L LY294002; (g–i) IEC-6 cultured with 10 μmol/L MK-2206 2HCL; 0 mmol/L boron (a, d, g), 0.8 mmol/ L boron (b, e, h) and 40 mmol/L boron (c, f, i) (B) The apoptosis rate of IEC-6 cells before and after LY294002 addition; (C) The apoptosis rate of IEC-6 cells before and after MK-2206 2HCL addition; Values are presented as the mean ± SD; Mean values with asterisk (*) differed significantly (p < 0.05), mean values with two asterisks (**) indicate extremely significant differences (p < 0.01).

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