Figure 2

ICG fluorescence imaging conditions. Correlation analysis revealed a positive association between protein concentration and fluorescence intensity ratio (Kendall’s tau-b = 0.828, P < 0.0001) (E). The fluorescence intensity ratio of A1 group was significantly different from that of A2 group (P < 0.05) (E). Fluorescence intensity ratio: B was used as the reference well, and the fluorescence intensity ratio was calculated by comparing the fluorescence intensity of A with that of B. (A) a mixture of albumin and ICG (concentration: 0.01 mg/ml) with varying protein concentrations (A1: 0.1 g/ml, A2: 0.01 g/ml, A3: 0.001 g/ml, A4: 0.0001 g/ml, A5: 0.00001 g/ml, A6: 0.000001 g/ml). (B) a mixture of albumin (concentration: 0.01 g/ml) and ICG (concentration: 0.01 mg/ml). (C) a mixture of saline and ICG (concentration: 0.01 mg/ml). (D) a mixture of bile and ICG (concentration: 0.01 mg/ml).