Figure 1 | Scientific Reports

Figure 1

From: Establishment of a forward primers-superposed amplification analysis for accurate aspirin pharmacogenomic measurement

Figure 1

Amplification plots of the triplex amplification analysis. The wild-type F-primer-based amplification assay and the mutated-type F-primer-based amplification assay were used to detect wild-type, mutated-type and heterozygous gDNA. (A) Amplification plots of un-optimized triplex amplification analyses. (B) Amplification plots of optimized triplex amplification analyses. Represented amplification plots are shown. The reaction was run in duplicate, in which 10 ng genomic DNA was inputted. Wt gDNA, wild-type genomic DNA; Mut gDNA, mutated-type genomic DNA; Het gDNA, heterozygote genomic DNA; WF primer, wild-type forward primer; MF primer, mutated-type forward primer.

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