Figure 2
Robustness assessment of the triplex amplification analysis. The robustness assessment was executed by employing mismatch allele-specific forward primers targeting single nucleotide polymorphism to simultaneously detect heterozygotes for PEAR1 (rs12041331), GP1BA (rs6065) and LTC4S (rs730012). Serial dilutions of heterozygote (1.25–40 ng) were measured by the triplex amplification analysis. Reactions were run in duplicate with three independent experiments. (A) Standard curve of the triplex amplification analysis. Amplification efficiency (Eff) % and R2 are shown. Data are expressed as mean ± SE. (B) Amplification plots of the robustness assessment. Represented amplification plots are shown. WF primer, wild-type forward primer; MF primer, mutated-type forward primer.
