Figure 4
From: CPT1A as a potential therapeutic target for lipopolysaccharide-induced acute lung injury in mice
Effects of CPT1A on LPS-induced apoptosis in MLE12 cells in vitro. (A) Caspase-3 activity in MLE12 cells treated with Lenti-CPT1A was determined. (B, C) The apoptosis rates of MLE12 cells treated with Lenti-CPT1A were determined by flow cytometry. (D, E) Bcl-2 and Bax mRNA in MLE12 cells treated with Lenti-CPT1A were determined. (F–H) BCL-2 and BAX protein in MLE12 cells treated with Lenti-CPT1A were determined. (I) Caspase-3 activity in MLE12 cells treated with etomoxir (10 μM) was determined. (J-K) Bcl-2 and Bax mRNA in MLE12 cells treated with etomoxir (10 μM) were determined. n = 4, *P < 0.05, **P < 0.01, and ***P < 0.001.
