Figure 3

Overexpression of hBcl2 protein can enhance the anti-apoptotic ability of BMSCs in vitro. (A) The CCK8 method was used to detect cell proliferation in hBcl2, cb, NC, and BMSCs groups at 1, 2, 3, 4, 5, 6, and 7 days (At least three independent replicates were performed for each experiment. hBcl2 comparisons are shown in red, cb comparisons are shown in gold, BMSC comparisons are shown in black, and NC comparisons are shown in blue. Data are presented as mean ± SD, ***P < 0.001, **P < 0.01, *P < 0.05 vs. BMSC group; ^###P < 0.001, ^##P < 0.01 vs. NC group, analyzed by two-way ANOVA, followed by Tukey’s post hoc test). (B) The CCK8 method was used to detect cell survival in each group at 1, 2, 3, 4, 5, 6, 7 and 8 days by using serum deprivation treatment (At least three independent replicates were performed for each experiment. hBcl2 comparisons are shown in red, cb comparisons are shown in gold, BMSC comparisons are shown in black, and NC comparisons are shown in blue. Data are presented as mean ± SD, **P < 0.01, *P < 0.05, vs. BMSC group; ^###P < 0.001, ^##P < 0.01, ^#P < 0.05 vs. NC group, analyzed by two-way ANOVA, followed by Tukey’s post hoc test). (C) The apoptosis of cells in each group was measured by TUNEL staining. DAPI (blue) stained nuclei indicating the total number of cells, and TUNEL (red) indicates the apoptotic cells. (D) The proportion of TUNEL positive cells in each group (At least three independent replicates were performed for each experiment. ****P < 0.0001 vs. hBcl2 group, analyzed by one-way ANOVA, followed by Tukey’s post hoc test). Scale bar = 50 μm.