Figure 4
Kcnj10-ZsGreen transgene expression in the adult mouse cochlea. (a) Cross-section of the stria vascularis (SV) showing KCNJ10 protein localization at the plasma membrane (red) surrounding ZsGreen cytoplasmic fluorescence (green). (b) High magnification view of a cross-section of the SV demonstrating co-expression of ZsGreen (green) and Kcnj10 mRNA detected by smFISH (red dots) in the same cells, indicative of the expression of ZsGreen in the intermediate cells. (c) Same image as (b) showing presence of Kcnj10 mRNA detected by smFISH (red dots) in intermediate cell nuclei and cytoplasm. (d) In the organ of Corti, ZsGreen fluorescence (green) was present in inner phalangeal cells, pillar and Deiters’ cells, identified by their acetylated tubulin immunolabeling (cyan). Inner hair cells (IHCs, arrowhead) and outer hair cells (OHCs, bracket) identified by their MYO7A immunolabeling, did not show ZsGreen fluorescence. Additionally, some faint signal was seen in regions corresponding to the inner sulcus/border cells and Hensen’s cells. (e) Cross-section of the modiolus showing ZsGreen fluorescence (green) in cells expressing SOX10, a satellite glial cell nuclear marker (blue). ZsGreen expression was not found in the spiral ganglion neurons immunolabeled with TUJ1 (red). (f) Same confocal acquisition as (e) showing that ZsGreen fluorescence did not overlap with TUJ1 labeling. Cell nuclei were labeled with DAPI. Imaging from P60 mice.
