Figure 1

Effects of existing drugs on serum starvation-induced Atrogin-1 expression in C2C12 myoblasts. (a) C2C12 cells were cultured for 72 h in DMEM with 2% horse serum to induce myotube formation. Subsequently, myotubes were cultured with or without (serum starvation) 2% horse serum for 24 h and stimulated with indicated drugs (each 1 µM) or 10 or 50 ng/ml rhIGF-1 for the last 6 h. Atrogin-1 expression was analyzed by realtime PCR. Data represent mean Atrogin-1 expression relative to Gapdh ± SD. Serum starvation without drugs or IGF-1 served as controls (each n = 3, *P < 0.05, ***P < 0.001 by Student’s t-test). (b) C2C12 cells were cultured 24 h in DMEM with or without (serum starvation) 10% FBS in the presence or absence of indicated concentrations of drugs No.001 or 003 for the last 6 h. Atrogin-1 expression was then analyzed by realtime PCR. Data represent mean Atrogin-1 expression relative to Gapdh ± SD. Serum starvation without drugs served as controls (each n = 3, *P < 0.05, ***P < 0.001 by Student’s t-test).