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Figure 1

From: Power-Doppler-based NH002 microbubble sonoporation with chemotherapy relieves hypoxia and enhances the efficacy of chemotherapy and immunotherapy for pancreatic tumors

Figure 1

Contrast-enhanced ultrasound characterization for murine orthotopic UN-KC-6141 tumors. (A) Immunofluorescence staining of UN-KC-6141 tumor. Sections were stained with CD31 (red) and Hoechst33342 (blue). Tumor and normal tissue regions (yellow dashed line indicated tumor region) were separated and calculated for MVD (n = 3). A two-tailed unpaired t-test was used. **:P < 0.01. (B) The ultrasound image of the day 13 tumor-bearing mice. B-mode image (left), Contrast-enhanced ultrasound (CEUS) image (right) via NH002. Pancreas (incarnate color), Spleen (red color), Aorta (yellow color), Primary tumor (blue color), and Secondary tumor (green color). (C) The time-dependent intensity curve of different regions circled in (B) after 50 μl of 3 × 108 microbubble injection. (D) Representative diagrams of each region at 5, 15, 30, 45, and 120 s post microbubble injection. (E) The ultrasound image of day 10 tumor-bearing mice, B-mode image (left, blue, and yellow arrow = suspicious area), and CEUS image (right, blue arrow = tumor). (F) Tissue images were taken after mice were euthanized (blue circle = tumor) after the treatment. (G) H & E staining of pancreas tissue with tumor (blue circle). Scale bar = 0.25 cm.

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