Figure 1 | Scientific Reports

Figure 1

From: Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues

Figure 1

Expression of Cor a 1 genes in different hazel tissues. The RT-PCR products using gene specific primers to determine Cor a 1 mRNA expression were loaded on 1.5% agarose gels. In case no band was visible, a gel slice at the corresponding height was cut out. The purified mRNA was then used for the 2nd PCR. F: female flower; iN: immature nut; N: mature nut; C: catkin; P: pollen; ((+)) very low, (+): low, +: moderate, ++: strong expression levels. The RT-PCR samples used for cloning of the genes into expression vectors are marked with *M: 100 bp DNA standard (New England Biolabs; Frankfurt, Germany). 18S rRNA isolated in a similar fashion was used as a control.

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