Figure 2

Physicochemical characterization of Cor a 1 isoallergens. (A) CD spectroscopy. CD spectra at 25 °C (blue curve), after heating to 95 °C (red curve), followed by stepwise cooling to 25 °C (green curve). (B) CD melting curves. CD denaturation (red) and renaturation (black) curves were recorded at 217 or 220 nm in 10 mM Na₂HPO₄/NaH₂PO₄ pH 7.0. The T_m value is displayed on top of each spectrum. No Tm could be calculated for Cor a 1.0801. (C) ¹H-NMR-spectroscopy. Spectra of Cor a 1.0302 (160 µM), Cor a 1.0501 (200 µM), Cor a 1.0601 (60 µM), Cor a 1.0701 (190 µM) and Cor a 1.0801 (70 µM) were recorded in a Bruker Avance 600 MHz spectrometer at 295 K in 10% (v/v) D₂O, 10 mM Na₂HPO₄/NaH₂PO₄, pH7.0.