Figure 5

Cellular metabolism involved in the production of formate including reactions found responsible for host-microbiota co-metabolism. Dotted lines represent diet constituent involvement in the overall formate production, circled reactions when deleted together largely reduced the formate urinary production. Metabolites names (abbreviations are given in VMH IDs, www.vmh.life,⁶⁹): 10fth 10-formyl-THF; 3 pg 3-phosphoglyceric acid; chol choline; cys_L L-cysteine; dmgly dimethylglycine; fald formaldehyde; for formate; glc_D D-glucose; gly glycine; glyb betaine; glyc glycerol; glyc3p glycerol-3phosphate; gthrd GSH; his_L L-histidine; lac_D D-lactate; lkynr L-kynurenine; meoh methanol; methf 5,10-methenyl-THF; mlthf 5,10-methylene-THF; mma methylamine; orn ornithine; sarcs sarcosine; ser_L L-serine; thf tetrahydrofolate; trp_L L-tryptophane; tyr_L L-tyrosine. Reactions’ names can be found in Table S07. Complete KO analysis can be found in Table S08. Differential gene expression (DEG) and differential protein abundance (DEP) analysis of association to AD-related phenotypes can be found in Table S09.