Figure 1 | Scientific Reports

Figure 1

From: A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos

Figure 1

Instrument response function (IRF) characterization. (A) Example IRFs for three pixels in the SPAD array were plotted in dashed lines (blue: pixel [3, 140], red: [41, 140], yellow: [125, 140]) on a logarithmic vertical axis. They were spaced up to \(\approx \text {3.5 ns}\) apart. The IRF shapes were filtered by a Gaussian filter with a kernel size of nine TDC bins (solid lines) and fitted with Gaussian models (dotted lines). The colored triangles highlight the peak positions of the IRF models. (B) The same as (A), but on a linear vertical axis, with the model IRFs translated to a common point in time as a result of the delay correction. (C) 2D map of measured IRF peak positions across the SPAD array. (D) Vertical and horizontal average projections (blue) of IRF peak positions were overlaid over their standard deviations range (gray). (E) 2D map of IRF peak positions after the correction. (F) Vertical and horizontal average projections (blue) and standard deviations (gray) of the corrected IRF peak positions.

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