Figure 5 | Scientific Reports

Figure 5

From: A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos

Figure 5

Autofluorescence lifetime imaging stills of algae. (A–B) Transmission images of the microalgae are in the top row. Their fluorescence lifetime contrast images are in the bottom row. The fluorescence lifetime contrast range (0.5–1.6 ns) is the same in all four panels with the palette colorbar displayed in (A). The crosses show the positions of representative pixels, for which the decays are in (E, F). D. quadricauda are in (A, B, E). C. vulgaris are in (C, D, F). Samples (A, C) are the control cells that were only treated with DMSO and exhibit a shorter lifetime. Samples (B, D) were incubated with the photosynthesis inhibitor DCMU in DMSO and display a longer lifetime. (E, F) The representative fluorescence decays of control cells (dotted lines) and DCMU-treated cells (solid lines) are displayed with the measured IRF (black line, scaled to range). (G) Normalized histograms show the fluorescence lifetime distributions in the non-zero pixels of the images. Scale bar \(\text {10}\,\upmu \text {m}\). Reproduced from76 with permission.

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