Figure 8

Selective plane illumination fluorescence lifetime volume images of GUVs. (A, B) Panels are made of three columns titled at the top. The first column contains URL links to image stack fly-through videos of immobilized GUVs. The second column contains links to 3D volume render videos of the same image stacks. The third column contains representative images from the stacks with crosses highlighting the points from which the representative decays were produced (C). (A) Videos and an image of a GUV cluster prepared from a DPPC/cholesterol mixture. The di-4-ANEPPDHQ in this membrane exhibits a longer fluorescence lifetime (\(\approx \text {2.7 ns}\)). (B) Videos and an image of a GUV prepared from DOPC. The di-4-ANEPPDHQ in this membrane exhibits a much shorter fluorescence lifetime (\(\approx \text {1.1 ns}\)). (C) Representative fluorescence decays taken from the same coordinate (marked by a cross) in the images in (A, B). The decays contain 5670 counts (A) and 1417 counts (B), respectively. (D) Histograms of fluorescence lifetime distribution in the 3D stacks of images in (A, B). Scale bars \(\text {10}\,\upmu \text {m}\). Numbers in the lower-right corners of the videos and images mark the relative axial position of each image stack.