Figure 3
From: HSPA9 reduction exacerbates symptoms and cell death in DSS-Induced inflammatory colitis
HSPA9 downregulation aggravates colonic histopathology and increases cytokine production in DSS-induced colitis. (A) To elucidate the impact of HSPA9 downregulation in DSS-induced colitis, we performed histological evaluation of colon tissue. Hematoxylin & eosin (H&E) staining shows structural integrity and immune cell infiltration while Periodic acid–Schiff (PAS) staining revealed the status of mucin-secreting goblet cells. The expression level of ZO-1, a tight junction marker, was visualized through immunohistochemistry. (B) Inflammatory cytokine levels including IL6, TNFA, and IFNG, in colonic tissue were evaluated using qRT-PCR. Data are presented as the mean ± standard deviation. Statistical analysis was performed with a two-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.0001.
