Figure 3 | Scientific Reports

Figure 3

From: Nortriptyline hydrochloride, a potential candidate for drug repurposing, inhibits gastric cancer by inducing oxidative stress by triggering the Keap1-Nrf2 pathway

Figure 3

NTP destroys the cell membrane and induces apoptosis in GC cells. (A,B) Fluorescence images of AGS and HGC27 cells after 24 h of NTP treatment stained with Hoechst 33342/PI, with blue representing the nucleus and red representing the PI infiltrating the cells. (C–E) AGS and HGC27 cells underwent apoptosis after treatment with NTP for 24 h, as did the percentages of cells undergoing early and late apoptosis. (F–H) Western blotting was used to measure the expression of the apoptotic proteins Bcl2, BAX, BDA, PARP and C-PARP in AGS and HGC27 cells treated with NTP for 24 h. GAPDH levels were used in a quantitative measurement of protein expression levels. Data are shown as the mean ± SD, with significant differences from the control group marked by *p < 0.05, **p < 0.01, ***p < 0.001.

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