Figure 5 | Scientific Reports

Figure 5

From: Nuciferine reduces vascular leakage and improves cardiac function in acute myocardial infarction by regulating the PI3K/AKT pathway

Figure 5

Nuciferine upregulated the phosphorylation levels of the PI3K/AKT pathway. (A) Heatmap of differential genes, data set GSE147365, including 6 control samples and 6 heart tissues from mice with acute myocardial infarction. (B) KEGG enrichment analysis of differential genes. (C) Transwell assay was performed to detect the effects of different concentrations of wortmannin (0, 10 nM, 20 nM, 50 nM, 100 nM, 200 nM) on HCMECs permeability. n = 3. (D) Western blotting was performed to detect the effect of nuciferine on the expression of related proteins in the myocardial tissues. (E, F) Quantification of the phospho-PI3K and phospho-AKT expression. n = 4. (G) Western blotting to detect the expression of related proteins under OGD conditions in HCMECs. (H, I) The quantification of the phospho-PI3K and phospho-AKT expression. n = 4. Data are expressed as the mean ± SD. NS No significance (P > 0.05); *P < 0.05, **P < 0.01, ***P < 0.001 vs. Normal or indicated group.

Back to article page