Figure 6 | Scientific Reports

Figure 6

From: Nuciferine reduces vascular leakage and improves cardiac function in acute myocardial infarction by regulating the PI3K/AKT pathway

Figure 6

Nuciferine protects the endothelial barrier by regulating the PI3K/AKT pathway. (A) Western blotting was performed to detect the effect of nuciferine on the expression of related proteins (in the presence or absence of wortmannin) in HCMECs. (B, C, D) The quantification of the phospho-PI3K, phospho-AKT expression, and intracellular Ve-cadherin. n = 4. (E) Representative images of Ve-cadherin immunofluorescence staining of HCMECs under each of the indicated experimental conditions. Scale bar = 10 μm. (F) Quantitative analysis of the intracellular Ve-cadherin fluorescence intensity of HCMECs. n = 4. (G) The quantification of the permeability of HCMECs under each of the indicated experimental conditions. n = 4. Data are expressed as the mean ± SD. NS, No significance (P > 0.05); *P < 0.05, **P < 0.01 vs. Ctrl or indicated group.

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