Figure 1

Class 3 semaphorin expression levels in wild-type versus altered FOXA1 prostate cancer specimens using cBioPortal. Boxplots of class 3 semaphorin mRNA levels in wild-type (shaded dark) versus altered FOXA1 specimens (shaded light) from the Prostate Adenocarcinoma cohort (TCGA, PanCancer Atlas) (a) and the Metastatic Prostate Adenocarcinoma cohort (SU2C/PCF Dream Team, PNAS 2019) (b) obtained from cBioPortal. Boxplots of class 3 semaphorin mRNA levels in the TCGA (c) and the SU2C/PCF (d) cohorts as a function of FOXA1 class variants. Class-1 variants included those in the FKHD (residues 168 to 269); class-2 variants included all variants c-terminal to residue 269; ‘Spans both’ in the SU2C/PCF cohort includes one or more variants that span residues used to define both class-1 and class-2 variants; ‘Neither’ indicates a variant in FOXA1 that was outside of the definition of both class-1 and class-2 variants. The number of samples are as follows: for TCGA, WT n = 461, Class 1 n = 25, Class 2 n = 1, Neither n = 1; for SU2C/PCF, WT n = 187, Class 1 n = 9, Class 2 n = 6, Spans both n = 4, Neither n = 2. Boxes span the interquartile range. Horizontal line within the box represents the median SEMA3C mRNA levels. Bars represent the 95th percentile range of readings. Statistical analysis was performed using the unpaired two-tailed Student’s t-test relative to WT samples. TCGA, mRNA expression z-scores relative to diploid samples (RNA Seq V2 RSEM). SU2C/PCF 2019, mRNA expression z-scores relative to diploid samples (FPKM capture).