Table 2 HPLC-based method validation data for CS leaf extracts and marker compounds.

From: Chemoprofiling and medicinal potential of underutilized leaves of Cyperus scariosus

Compound

λmax (nm)

Linearity Equation

Determination Coefficient (R2)

Linearity range (ng/µL)

LOD (ng/µL)

LOQ (ng/µL)

Linearity and sensitivity

 Caffeic acid

326

y = 61.84x − 27.62

0.9998

1–60

0.32

0.98

 Catechin

280

y = 5.23x − 3.73

0.9998

5–60

1.63

4.95

 Epicatechin

280

y = 4.61x − 3.84

0.9997

5–60

1.64

4.97

 Trans-p-coumaric acid

326

y = 63.20x − 27.91

0.9998

1–60

0.30

0.91

 Trans-ferulic acid

326

y = 60.17x − 25.69

0.9998

1–60

0.33

0.99

Compound

Amount (ng/µL)

Precision (% RSD)

Intra-day (n = 3)

Inter-day (n = 3)

 

RT (min)

Peak area

RT (min)

Peak area

Precision

 Caffeic acid

10

0.14

0.29

0.70

2.31

 

20

0.03

0.75

0.69

1.22

 

40

0.14

0.62

0.52

0.24

 

 Catechin

10

0.17

1.76

1.09

2.26

 

20

0.04

1.74

1.08

2.40

 

40

0.18

0.42

0.84

0.32

 

 Epicatechin

10

0.15

1.71

0.85

2.16

 

20

0.02

1.33

0.86

2.04

 

40

0.09

0.86

0.67

1.01

 

 Trans-p-coumaric acid

10

0.14

0.26

0.53

1.48

 

20

0.02

0.68

0.52

0.80

 

40

0.06

0.67

0.39

0.21

 

 Trans-ferulic acid

10

0.08

0.22

0.35

1.36

 

20

0.02

0.48

0.22

0.15

 

40

0.09

0.61

0.27

0.44

 

Compound

% Recovery

Spike level-1

Spike level-2

Spike level-3

 

Accuracy

 

 Caffeic acid

104.32

106.51

103.48

   

 Catechin

96.21

99.63

97.52

   

 Epicatechin

98.73

96.86

94.37

   

 Trans-p-coumaric acid

103.26

104.27

106.77

   

 Trans-ferulic acid

104.75

102.83

103.28

   
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