Figure 2
From: LncRNA GAS5 restrains ISO-induced cardiac fibrosis by modulating mir-217 regulation of SIRT1
Overexpression of GAS5 suppressed the expression of mir-217 and promoted the expression of SIRT1 both in vivo and in vitro. (A) To prove that the overexpression of GAS5 suppressed the expression of mir-217 and promoted the expression of SIRT1 in vivo, WB and RT-qPCR were performed to explore the expression of SIRT1, and PCR was performed to explore the expression of mir-217. (B) To prove that the overexpression of GAS5 suppressed the expression of mir-217 and promoted the expression of SIRT1 in vitro, MCFs were transfected with AAV9-GAS5 or AAV9-NC. Then the expression of GAS5 and mir-217 in MCFs was detected by RT-qPCR; the expression of SIRT1 was detected by RT-qPCR and WB. (C) Protein expression of collagen I, collagen III and α-SMA showed that the overexpression of GAS5 alleviated fibrosis of MCFs induced by TGF-β1. (D) Protein expression of NLRP3, Caspase-1, IL-1β and GSDMD showed that the overexpression of GAS5 suppressed pyroptosis in the TGF-β1-treated MCFs. *p < 0.05, **p < 0.01, ***p < 0.001. Data are represented as mean ± SEM, n = 6.
