Figure 3
From: LncRNA GAS5 restrains ISO-induced cardiac fibrosis by modulating mir-217 regulation of SIRT1
mir-217 aggravated fibrosis via down-regulating the expression of SIRT1 but stimulating NLRP3 inflammasome activation-mediated pyroptosis in vitro. (A) MCFs were transfected with mir-217 mimics and inhibitor. Then the expression of mir-217 in MCFs was detected by RT-qPCR. (B) Protein expression of collagen I, collagen III and α-SMA in MCFs was detected by WB. (C) Protein expression of NLRP3, Caspase-1, IL-1β and GSDMD in MCFs was detected by WB. (D) Protein and mRNA expression of SIRT1 in MCFs was detected, and immunofluorescence staining of SIRT1(red) was performed in MCFs at × 200 magnification. *p < 0.05, **p < 0.01.**P < 0.01, ***P < 0.01. Data are represented as mean ± SEM, n = 6.
