Figure 4
From: LncRNA GAS5 restrains ISO-induced cardiac fibrosis by modulating mir-217 regulation of SIRT1

LncRNA GAS5 alleviateed cardiac fibrosis induced by NLRP3 inflammasome activation-mediated pyroptosis via regulating mir-217/SIRT1 pathway. The MCFs were co-transfected with GAS5 overexpression plasmid and mir-217 mimics or inhibitor, respectively, then were treated with TGF-β1. Then the protein expression of collagen I, collagen III and α-SMA (A); the protein expression of NLRP3, Caspase-1, IL-1β and GSDMD (B); and the protein and mRNA expression of SIRT1 (C) in MCFs were detected. *p < 0.05, **p < 0.01.**P < 0.01, ***P < 0.01. Data are represented as mean ± SEM, n = 6.