Figure 6

(A) Gene expression analysis for dIlp2 mRNA expression in head samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), LaminCa/αTub84B or LaminCa/αTub84B/eEF1α1. Two-way ANOVA was performed with effects as follows: normalization; p < 0.0001; sex; ns and their interaction; ns with a Sidak’s multiple comparisons test comparing dIlp2 expression between males and females. (B) Gene expression analysis for dIlp3 mRNA expression in head samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), LaminCa/αTub84B or LaminCa/αTub84B/eEF1α1. Two-way ANOVA main effects as follows: normalization; p = 0.0032; sex p < 0.0001 and their interaction; ns with a Sidak’s multiple comparisons test comparing dilp3 expression between males and females. **p < 0.005. (C) Gene expression analysis for dIlp5 mRNA expression in head samples of Canton S10 (CS) or Dahomey (Dah) flies, 2 days (D2) or 7 days (D7) post eclosion in male and female flies. Gene expression was normalized using ribosomal genes as reference genes (RpL32/RpS13), LaminCa/αTub84B or LaminCa/αTub84B/eEF1α1. Two-way ANOVA main effects as following: normalization; p < 0.0001; sex p < 0.0001 and their interaction; ns with a Sidak’s multiple comparisons test comparing dilp3 expression between males and females.