Table 2 Primer sequences and primer efficiency.
From: Reference genes to study the sex-biased expression of genes regulating Drosophila metabolism
Gene name | Forward primer (5'-3') | Reverse primer (5'-3') | Efficiency head | Efficiency body | Efficiency whole fly |
|---|---|---|---|---|---|
Act42A | TGCAAAAGGAAATCACGGCG | CCGCCGATCCAAACAGAGTA | 97 | 105 | Â |
Arp3 | ACTACTTCCTGCTGACTGAG | CCTGGACGGCGATATACA | 132 | 93 | Â |
aTub84B | GGAGTTCGCCATCTACCCAG | ACTGATTTCGACGGTTACCCC | 83 | 78 | Â |
bTub60D | CAAATCGGCGCTAAGTTCTGG | CCCACGTAGATGCCATTGCT | 91 | 109 | Â |
bTub97EF | CCGCATCATGAACTCCTTCT | TGGACAGCGTGGCATTGTAC | 119 | 94 | Â |
eEF1a1 | AGGCCGCCTAAATTGGGAAA | ATCGGAGGCAACAAGCAAA | 98 | 152 | Â |
LaminCa | TCCACCCAACAATCTGGTGAT | GCAACATCCTCTTTGTCGGC | 94 | 100 | Â |
RpL32 | GTCCCAAGGGTATCGACAACA | CTTGCGCTTCTTGGAGGAGA | 95 | 102 | Â |
RpS13 | GGGTCTGAAGCCCGACATT | GGCGACGGCCTTCTTGAT | 93 | 96 | Â |
dIlp2 | AGCAAGCCTTTGTCCTTCATCTC | ACACCATACTCAGCACCTCGTTG | 93 | Â | Â |
dIlp3 | GCAATGACCAAGAGAACTTTGGA | GCAGGGAACGGTCTTCGA | 93 | Â | Â |
dIlp5 | GCTCCGAATCTCACCACATGAA | GGAAAAGGAACACGATTTGCG | 94 | Â | Â |
Bmm | AATGGCGTCGAATCAGACTT | AACACAGATGGGGATTTGGA | Â | 126 | Â |
dIlp6 | TGCTAGTCCTGGCCACCTTGTTCG | GCTTCCCGAAACTGTTGGGAAATA | Â | 128 | Â |
Fit | TCGTTGGTCTGAGGAGGACA | CCAGTTGACAGAGTGCGGAT | Â | 105 | Â |
Foxo | AAATTCGTCTATCGGCTGCG | TTGGAAGATAATAACTGCGCCTCT | Â | 106 | Â |
Drs | ACCAAGCTCCGTGAGAACCTT | TTGTATCTTCCGGACAGGCAG | Â | 109 | Â |
Lpin | ATCCCACGTCCCTGATATCG | TTCATCTTGGTTGGTTAGCAGG | Â | Â | 104 |
FASN1 | GTTGGGAGCGTGGTCTGTAT | GCACACCGAAGAACTGTTGG | Â | Â | 97 |
ACc | GGCTATGCTGCGCTTAACA | GCCTCTGTTTTGTGGGTGAC | Â | Â | 85 |
HSL | GATCCATTCCTGTCGCCGTA | CAGGGGTCCATGTTAAGTGTAAGT | Â | Â | 102 |
LSD1 | TCACAATCTCACGGCTGGAC | GGCTACCATAGAACGCCAGC | Â | Â | 112 |
LSD2 | CGGATGTCGAGGAAGATAATGATGA | GTCGTAGCTCTCCCCAACAA | Â | Â | 128 |
ATPCL | CTTCTGACCATCGGGGATCG | CAGGTTGGTGTCGTATGCCT | Â | Â | 95 |
Sea | ACATGAAGGAGCTGGGCGTC | CCAGGGTCTCCTCGTCGAAC | Â | Â | 93 |
Pgi | GCATTCCAAGGAGCCTGAGT | ATGTTGGTGCTGTCAATGCC | Â | Â | 111 |
Tpi | CATCAAGGACCTGTTCGTGAA | AGTCCAGCAGTATCTCGCCAT | Â | Â | 103 |
PEPCK | GCCTGAGCTCATTGAACAAAG | ACCGCAATTGTCCTGGCGCA | Â | Â | 101 |
bigmax | GCCAAGTTTCAAGTGTTCCAG | CTCCAGCCAGGGGATAATG | Â | Â | 112 |
GADPH2 | ATGAAATTAAGGCCAAGGTT | GAGTAGCCAAACTCGTTGTC | Â | Â | 102 |
GADPH1 | CTCGACTCACGGTCGTTTCA | GGTGATCTTCTGGCCGTTCA | Â | Â | 59 |
Upd2 | AGTGCGGTGAAGCTAAAGACTTG | GCCCGTCCCAGATATGAGAA | Â | Â | 83 |
zw | ATCTGTGCGGGAAGTGATG | CCAAACTTGGCTTCGGAAC | Â | Â | 84 |
Pgd | ATGAGCGGACAAGCGGATATT | TAGGCGCACACCACGAATC | Â | Â | 98 |
GlyS | AGTCCTACTTCATCGCGGCA | GTCTCCTCATCCACGGAGTC | Â | Â | 81 |
GlyP | ATACAACAACAACCACGTAAACAC | GGATGTAGTCACCATCGTTGAAG | Â | Â | 97 |
Tps1 | TTCCTGGACATCCCATTCCC | TCACAACCCAACATACCCTGT | Â | Â | 105 |
Eiger | TCCTAGTCCGCAAAGGTGAA | CAAGTGGAAATGGGCTGCTG | Â | Â | 82 |
TACE | AGAATTGCGCGGCCCTG | CTTGGCACCCCTTTTCACCA | Â | Â | 103 |
cchamide | CGCCAAATGAACAGGTGCC | GTCGGCGAGGTCGGTTAAA | Â | Â | 104 |
Pgk | TCATCGTGTGGAACGGACCC | AGACGAGTGCCTCCGTGTTC | Â | Â | 90 |
Rel | TTCCGTGAAAAGCTGACCCG | GAGTGGGCTGGTGAAAAGGA | Â | Â | 104 |
STAT92E | CAGGGCGTTCTCTGACCATT | TTCGGGCTCTTGACGCTTTG | Â | Â | 100 |
