Figure 5
MAPK is a target gene of miR145-5p, and the effects of miR-145-5p overexpression and treadmill training on lung function in SCI rats. (A) Predicted sequence of miR145-5p and binding site of MAP3K3. (B) Dual luciferase reporter gene assay. (C) EGFP fluorescent labeling assay, scale bar = 100 µm. (D) miR145-5p relative expression levels. (E) Western blotting detection of MAP3K3, Erk. (F) Relative protein expression levels of MAP3K3, Erk. (G) HE staining results of Lung tissues, scale bar = 50 µm. (H) Lung pathology scoring results, n = 6. (I) pH level, n = 6. (J) PaO2 level, n = 6. (K) PaCO2 level, n = 6. (L) Lung wet/dry weight ratio, n = 6. *P < 0.05 as determined by one-way ANOVA (Tukey’s multiple comparison test).
