Figure 6
Effect of overexpression of miR145-5p on the MAPK signaling pathway and lung cell apoptosis in rat lung tissues post-SCI. (A) Western blotting detection of MAP3K3, Erk expression. (B) Relative protein expression levels of MAP3K3, Erk. (C) Bax immunofluorescence staining, scale bar = 20 µm. (D) Bcl-2 immunofluorescence staining, scale bar = 20 µm. (E) p-Erk immunohistochemical staining, scale bar 50 = µm. (F) CytC immunofluorescence staining, scale bar = 20 µm. (G) caspase 9 immunohistochemical staining, scale bar = 50 µm. (H) Bax-positive cell rate, n = 3. (I) Bcl-2-positive cell rate, n = 3. (J) CytC-positive cell rate, n = 3. (K) caspase 3 immunohistochemical staining, scale bar 50 = µm. (L) p-Erk-positive cell rate, n = 3. (M) caspase 9-positive cell rate, n = 3. (N) caspase 3-positive cell rate, n = 3. (O–P) Western blotting detection of caspase 9, Bax, Bcl2, and CytC expression. (Q) Relative protein expression levels of caspase 9, caspase 3, Bax, Bcl-2, and CytC. (R) Relative expression levels of caspase 9, caspase 3, Bax, Bcl-2, CytC mRNA. *P < 0.05 as determined by one-way ANOVA (Tukey’s multiple comparison test).
