Figure 8
Effects of knockdown of miR145-5p on the MAPK signaling pathway and apoptosis in lung tissues of rats following SCI. (A) Immunohistochemical staining of p-Erk, scale bar 50 = µm. (B) Rate of p-Erk-positive cells, n = 3. (C) Western blotting detection of MAP3K3, Erk expression. (D) Relative protein expression levels of MAP3K3, Erk. (E) Rate of caspase 9-positive cells, n = 3. (F) Rate of caspase 3-positive cells, n = 3. (G) Immunofluorescence of Bax staining, scale bar = 20 µm. (H) Immunohistochemical staining of caspase 9, scale bar = 20 µm. (I) Immunofluorescence staining for Bcl-2, scale bar = 20 µm. (J) Immunohistochemical staining for caspase 3, scale bar = 50 µm. (K) Immunofluorescence staining for CytC, scale bar = 20 µm. (L) Rate of Bax-positive cells, n = 3. (M) Rate of Bcl-2-positive cells, n = 3. (N) Rate of CytC-positive cells, n = 3. (O)Western blotting detection of caspase 9, caspase 3, Bax, Bcl-2, and CytC expression. (P) Relative protein expression levels of caspase 9, caspase 3, Bax, Bcl-2 and CytC. (Q) Relative expression levels of caspase 9, caspase 3, Bax, Bcl-2, CytC mRNA. *P < 0.05 as determined by one-way ANOVA (Tukey’s multiple comparison test).
