Figure 1
From: Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter
Secretion of pro-HlyA by chHlyB ABC transporters. Immunoblot analysis of whole E. coli cells expressing the indicated chHlyB show the presence of pro-HlyA (a), chHlyB (b) and HlyD (c). SDS-PAGE analysis (d) shows secreted pro-HlyA in the supernatant of E. coli cells expressing the different chHlyB, HlyD and pro-HlyA. The contrast of the SDS-PAGE in (d) was adjusted to improve visibility of secreted pro-HlyA in the case of HlyB-EEK. Schematic representation of the chHlyB variants are depicted above the respective chimera. Blue domains originate from HlyB (E. coli) and red domains from RtxB (K. kingae). Cell and supernatant samples were diluted to match the same OD600. Note that samples marked with an asterisk are not from the same Western blot membrane. Uncropped Western blots and SDS-PAGE gels are shown in Supplementary Fig. S5. Signals corresponding to unspecific binding and degradation are marked in case of the anti-HlyD western blot. M: Protein marker, the approximated molecular weight of the marker proteins is given on the left; x h: time after induction, when the samples were taken.
