Figure 2 | Scientific Reports

Figure 2

From: Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter

Figure 2

Analysis of purified RtxB-NBD. (a) SDS-PAGE analysis of purified RtxB-NBD. Shown is the elution fraction (E) after Size Exclusion Chromatography with Coomassie staining. Chromatograms of the purification and an uncropped SDS-PAGE analysis of additional samples are shown in Supplementary Fig. S7. RtxB-NBD-NHis6 has a size of 27,676 Da. M: Protein marker, the approximated molecular weight of the marker proteins is given on the right. (b) ATPase activity of RtxB-NBD in dependency of varying ATP concentrations. The data was analyzed using the Hill equation. Shown are mean values and standard deviations as error bars of three independent experiments.

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