Figure 2
(A) Luciferase Assay analyzed the relative Luciferase Activity (NC, blue column; 3’UTR Vector, Red column). NS. non significant. (B) Luciferase Assay analyzed the relative Luciferase Activity (NC, blue column; h-SATB1-WT, Red column). (C) Luciferase Assay analyzed the relative Luciferase Activity (NC, blue column; h-STIM1-WT, Red column). All groups underwent three technical replicates. D. qRT-PCR analyzed miR641 expression in 293 T cells after miR641 over-expression (lentivirus transfection), (Vecter, blue column; miR641 over expression, Red column), *p < 0.05 . E. qRT-PCR analyzed SATB1 expression in 293 T cells after miR641 over-expression(lentivirus transfection), (Vecter, blue column; miR641 over -expression, Red column), *p < 0.05. F. qRT-PCR analyzed STIM1 expression in 293 T cells after miR641 over-expression (lentivirus transfection), (Vecter, blue column; miR641 over- expression, Red column), *p < 0.05 . G. Western blotting analyzed the expression SATB1 and STIM1 encoded proteins after miR641 over-expression (lentivirus transfection) in 293 T cells (Vector Left; miR641 over-expression Right). All groups underwent three technical replicates.
