Figure 2
DNA turnover, visual confirmation of regeneration, and gene expression of myogenic genes. (A) Visualization of muscle damage 4 days after CTX injection. Image taken at ×10 magnification. (B) Quantification of damaged muscle tissue as a fraction of whole tissue surface area. Values are expressed as a percentage (%) of damaged tissue area in µM2/whole tissue surface area in µM2. Significance determined by One-Way ANOVA with Benjamini–Hochberg correction for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. (C) Visualization of central nuclei expression 14 days after CTX injection. Image taken at ×10 magnification. (D) Quantification of individual myofibers expressing central nuclei. Values are expressed as a total number of myofibers with central nuclei per slide. Significance determined by One-Way ANOVA with Benjamini–Hochberg correction for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. (E) Measuring the fractional replacement rate (f-Value) of DNA with 2H in newly dividing myocytes during various stages of muscle regeneration. Significance was determined by One-Way ANOVA with Benjamini Hochberg correction for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. (F) Myogenic gene expression in all stages was measured in FPKM and compared to control group. Significance for each individual gene determined by Student’s Unpaired T-Test, *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.
