Figure 2
From: Developing centrifugal force real-time digital PCR for detecting extremely low DNA concentration
Schematic representation of the reagent partitioning process using centrifugal force. (a,b) Mixing of dPCR emulsion reagents. (c) Loading dPCR emulsion reagents into the 22Â k-Single-Disk. (d) Schematic representation of partitioning the dPCR emulsion reagent as it is spun in the 22Â k-Single-Disk. (e) Analysis of the partitioning ratio of all wells to total wells using metadata (21,399/22,000, 97.3%). (f) Scan images of the post-PCR cycle before reagent addition, after uniform partitioning, and after.
