Figure 4

Mdivi-1 induces detachment of breast cancer cells by a DRP1-independent mechanism. (a) DRP1 and β-actin protein levels in WT and DRP1-KO HCT116 cells were determined by western blot (b) these cells were treated with 50 μM mdivi-1 for 24 h. Next, the fraction of viable floating cells relative to the total viable cell population was determined. (c) MCF-7 and MDA-MB-231 cells were treated with 50 μM mdivi-1 for 4 h. Then, a Mito Stress test was performed using a seahorse XFe96 analyzer. (d) MCF-7 and (e) MDA-MB-231 cells were treated with 10 μM rotenone for 24 h, and then, the fraction of viable floating and adherent cells was determined. (f) MCF-7 and MDA-MB-231 cells were treated with 5 μM antimycin-A (AA) and 5 μM oligomycin (Oligo) for 24 h. The fraction of viable floating cells was determined. (g) 143Bwt, 143BΔcytb and (h) Rho-0 cells were treated with 50 μM mdivi-1 for 24 h. Then, the fraction of viable floating and adherent cells was determined. Results represent means ± SEM. *p < 0.05; **p < 0.01; ns = not significant.