Figure 3

Detection of transfection efficiency of cells and the influence of P4HA2 expression on the OSCC cells’ apoptosis and proliferation. (A) qRT-PCR and WB tests were used to measure the transfection efficiency of OSCC cells. (B) The CCK-8 test was used to measure the cell growth rates in the sh-P4HA2, sh-GFP, pcDNA3.1-p4HA2, and pcDNA3.1 groups of SCC-25 and SCC-9. The data indicated that P4HA2 could facilitate the proliferation of SCC-25 and SCC-9. (C) The cell cycle profile in sh-P4HA2, sh-GFP, pcDNA3.1-p4HA2, and pcDNA3.1 groups of SCC-25 and SCC-9 was investigated by flow cytometry. The data indicated that P4HA2 could affect the cell cycle by regulating the G1G0/S phase. (D) Apoptosis in the sh-P4HA2, sh-GFP, pcDNA3.1-p4HA2, and pcDNA3.1 groups of SCC-25 and SCC-9 was investigated through flow cytometry. The data indicated that inhibition of P4HA2 expression could accelerate cell apoptosis. (E) WB test was used to measure the protein expression of Cyclin D1 and survivin in the sh-P4HA2, sh-GFP, pcDNA3.1-p4HA2, and pcDNA3.1 groups of SCC-25 and SCC-9. The data indicated that P4HA2 knockdown suppressed the expression of Cyclin D1 and survivin proteins. Conversely, we observed opposite results when P4HA2 was overexpressed. N = 3. *:p less than 0.05, **:p less than 0.01, ***:p less than 0.001, ****:p less than 0.0001.