Figure 5 | Scientific Reports

Figure 5

From: P4HA2 promotes proliferation, invasion, and metastasis through regulation of the PI3K/AKT signaling pathway in oral squamous cell carcinoma

Figure 5Figure 5

P4HA2 Regulates the PI3K/AKT Pathway of OSCC Cells in a Collagen-Dependent Manner. (A, B) A WB test was used to measure the protein expression of collagen I, collagen IV, PI3K, p-PI3K, AKT, and p-AKT in the sh-GFP, sh-P4HA2, pcDNA3.1-p4HA2, and pcDNA3.1 groups of SCC-25 and SCC-9. The data indicated that the expression levels of collagen I, collagen IV, p-PI3K, and p-AKT in OSCC cells were notably reduced in the sh-P4HA2 group, while those in the pcDNA3.1-P4HA2 group were notably higher than those in the sh-P4HA2 group. The expression levels of PI3K and AKT did not change notably between the sh-P4HA2 group and the pcDNA3.1-P4HA2 group. (C) The effect of a P4HA2 enzyme agonist on the PI3K/AKT signaling pathway was determined by a WB assay in OSCC cell lines. N = 3. *:p less than 0.05, **:p less than 0.01, ***:p less than 0.001, ****:p less than 0.0001.

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