Figure 2

Multimodal imaging of a grade 1 invasive carcinoma of no special type (Category B) from a 51-year-old female. (A) Mammogram showing the biopsy cavity with gas locules in the right outer breast from known underlying malignancy (arrow) and the surrounding breast parenchyma consisting of scattered fibroglandular tissue (*) falling in BIRADS Category B. Patient underwent hookwire guided wide local excision. (B), Ex-vivo US (coregistered with PA images D and E) confirmed excision of the index breast cancer as represented by a markedly hypoechoic mass with slight irregular margins (arrow). Central echogenic focus within the mass represents the hookwire. (C) H&E stained microscopic image of tumour showing the presence of non-neoplastic breast tissue between the tumour (black arrow) and the scanned margin, comprising mostly adipose tissue (green dotted circled area) admixed with a minor component of fibrous tissue containing breast lobules and ductules (purple dotted boxed area). (D) PA image of the total hemoglobin (HbT) distribution map corresponding to (C) shows expected thin layer of blood on specimen surface and few tiny traversing blood vessels. (E) Composite PA image showing the distribution of lipid (green) and collagen (magenta). Collagen and lipid marked by purple dotted box and green dotted circle respectively correspond to the collagen and lipid marked by purple dotted box and green dotted circle on image (C). Scale bars C, 10 mm, scale bars (B, D-E), 5 mm.