Figure 3

mRNA and protein localization of LsMVP. In situ hybridization (a–c) and immunohistochemistry (d–f) were performed to localize LsMVP mRNA and protein expression in the Atlantic salmon louse. LsMvp mRNA expression was localized to (a) immature oocytes, (b) intestine and ovaries, and (c) the subcuticular tissue. LsMVP protein was localized to (d) the cement gland, (e) intestine, and (f) the subcuticular tissue. Sense probe (in situ hybridization) or secondary antibody only (immunohistochemistry) were used as negative controls and did not give any signals (left corner boxed images). Int intestine, iO immature oocyte, Cu cuticula, Sub subcuticular tissue, Ova Ovarium, Oo oogonia, En enterocytes, Lu gut lumen, Mi microvilli, C cement, Cg cement gland, O oocytes.