Figure 3
RNH1 attenuates LPS-induced TNFα secretion in PBMCs. Isolated PBMCs exposed to 50 ng/ml LPS in the presence or absence of 640 U/ml RNH1 for 4 h compared to unstimulated cells were analyzed regarding TNFα protein concentrations quantified using ELISA. Data are presented as column bar graphs of the mean ± SEM (n = 10). Repeated-measures one-way ANOVA followed by Tukey’s test was used for multiple comparisons. ***p < 0.001; ****p < 0.0001; RNH1, ribonuclease inhibitor 1; LPS, lipopolysaccharide; TNFα, tumor necrosis factor α; PBMCs, peripheral blood mononuclear cells.
