Figure 5
From: Biparatopic anti-PCSK9 antibody enhances the LDL-uptake in HepG2 cells
LDL-uptake assay. The LDL-uptake test in HepG2 cell models. The horizontal axis represents six different groups. The vertical axis represents mean fluorescence intensity (MFI) of the intracellular LDL-BODIPY, which reflects the levels of the LDL-c metabolism. The right two groups were respectively served as the blank control (BC, no addition of PCSK9 and its inhibitor) and negative control (NC, only addition of 0.1 μM PCSK9). The left four columns represent the groups of the Repatha, VHH-B11-Fc, VHH-H2-Fc and B11-H12-Fc respectively in three doses (0.375, 0.750 and 1.500 μM). LDL-c uptake percent was given on the right (←). “(+)” and “(−)” refers to the addition and absence of hPCSK9 or Abs, respectively. The data were expressed as mean ± SD. Statistical significance was determined using Student’s paired t-test. P < 0.05 was considered as statistically significant, compared with the negative control group (*P < 0.05, **P < 0.005, ***P < 0.0005, ns: not significant).
