Figure 4
From: Werner helicase interacting protein 1 contributes to G-quadruplex processing in human cells
WRNIP1 interacts with PIF1 DNA helicase in vivo. (a) In a protein fragment complementation assay, we used the split Venus reporter plasmid system. PIF1 was cloned in fusion with the N-terminal part, and WRNIP1 was cloned in fusion to the C terminal part of Venus protein. Only the co-transfection of V1-PIF1 and V2-WRNIP1 resulted in green fluorescence sign, demonstrating the association of these two proteins and the specificity of the experiment. (b) The co-transfection of V1-PIF1, V2-WRNIP1, and WRNIP1 shDNS, or PIF1 shDNA, as indicated is resulted in the disappearing of the green fluorescence sign, proving the specific interaction between WRNIP1 and PIF1.
