Figure 5

WRNIP1 cooperates with PIF1 in replication of G4 forming regions. (a). Quantification of G4 intensity in shCtrl, shWRNIP1, shPif1 and shPif1/shWRNIP1 HeLa cells. Where indicated, cells were treated with G4 ligand PhenDC3 (10 µM). Cells were collected and G4 intensity was measured in 100 nuclei per sample. The depletion of WRNIP1, Pif1 and Pif1/WRNIP1 was tested by Western blot (bottom). (b) Representative images from (a). Scale bars, 20 μm. (c) Quantification of γH2AX intensity in shCtrl, shWRNIP1, shPif1 and shPif1/shWRNIP1 HeLa cells. Where indicated, cells were treated with G4 ligand PhenDC3 (10 µM). Cells were collected and γH2AX intensity was measured in 100 nuclei per sample. (d) Representative images from (c). Scale bars, 20 μm. Error bars represent SEM of 3 biological replicates on graphs. p-values were obtained by ANOVA (Origin Pro) (*P < 0.05, **P < 0.01, and ***P < 0.001). Statistical data is provided in Table S4. Original Western blot membrane is showed in Supplementary Fig. 5.