Figure 3 | Scientific Reports

Figure 3

From: Zinc finger transcription factor ZFP1 is associated with growth, conidiation, osmoregulation, and virulence in the Polygonatum kingianum pathogen Fusarium oxysporum

Figure 3

Verification of zfp1 and zfp1 tZFP1. (A): Verification of Zfp1 by PCR. 1: Primer pair of Zfp1-IF/Zfp1-IR; 2: Hy-F/Yg-R; 3: Zfp1-UH-F/Zfp1-UH-R; 4: Zfp1-DY-F/Zfp1-DY-R. (B): Zfp1 amplification with Zfp1-IF/Zfp1-IR. (C): Hygromycin B-resistant gene amplification with Hy-F/Yg-R. (D): Neomycin-resistant gene amplification with Neo-F/Neo-R. (E): Relative expression of Zfp1 in ZFP1, zfp1, and zfp1 tZFP1. Error bars represent standard deviation of the mean with three independent biological replicates. Different letters indicate significant differences at P < 0.05.

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