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Figure 1

From: Divergent histopathological and molecular patterns in chemically induced interstitial cystitis/bladder pain syndrome rat models

Figure 1

Bladder tissue pathological assessment and quantification in interstitial cystitis rat models. (A) Flowchart showing the experimental design of the animal studies. (B) Histologic features of interstitial cystitis induction in bladder tissue were observed using H&E staining, and the infiltrated mast cells distributed in bladder tissues were stained with toluidine blue (arrows). Characterization of rat bladder sections using Masson’s trichrome staining; images show collagen with blue staining and muscle with purple staining. (C) The number of mast cells per mm2 in the bladder epithelium and lamina propria (n = 3). (D) The sum intensity of collagen in the bladder epithelium and lamina propria was determined based on the images stained with Masson’s trichrome taken using TissueFAX Plus software (n = 3–4). Bars depict the means ± SDs. There is no significant difference between bars with the same letters, and there is a significant statistical difference between bars with different letters determined using one-way ANOVA and a post-hoc test with Tukey’s HSD (honest significant difference) test.

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