Figure 1
Set-up of the murine liver perfusion model. (a–c) Schematic and representative image of the liver perfusion mouse model with corresponding timeline of the experiments and analysis pipeline. (d) Validation experiment for the IRDye 800-perfusion method to determine the percentage of sufficiently perfused liver tissue (perfusion rate). As control condition, the right hepatic branch of the portal vein was ligated during perfusion with the IRDye 800 indicated in the image on the left and liver tissue was dissociated and subjected to flow cytometry analysis. Results are presented as scatter blot with side scatter area (SSC-A) vs. IRDye 800 intensity. An unstained liver served as control to set the “non-perfused” gate. For all liver perfusion experiments included in the following figures, perfusion rate attained minimum 90%. (e) Validation experiment for quantification of liver cell damage via the enzymatic activity of alanine aminotransferase (ALT). Output samples were collected at the start of the experiment (baseline), 150 min after ligation (180 min after perfusion start) of the right branch of the hepatic portal vein (I), 1 min (II) and 10 min after re-opening of the ligation (III). ALT activity in all liver perfusion experiments included in the following figures were in the range of baseline levels confirming sufficient perfusion during the experiments.
