Figure 5
From: Stanniocalcin-1 promotes temozolomide resistance of glioblastoma through regulation of MGMT
STC1 knockdown enhanced the inhibition and apoptosis of cultured GBM cells induced by TMZ treatment. (A–B) Validation of STC1 knockdown in GBM cell lines by qRT-PCR and (C–D) Western blot. (E–F) Representative images (left panels) and quantification (right panels) of the colony formation assays on STC1-knockdown DBTRG (E) and U87-MG (F) cells treated with 200 μM TMZ (scale bar, 10 mm). (G–H) Cell viability and IC50 values of TMZ treatment for 72 h in DBTRG (G) and U87-MG (H) cells. (I) Images (left panels) and quantification (right panels) of flow cytometry on TMZ-induced apoptosis in DBTRG STC1-knockdown cells. *: p < 0.05, **: p < 0.01, ***: p < 0.001.
